ABI 3130 / 3130XL

The ABI 3130 and 3130xl Genetic Analyzers are in a range of analyzers produced by ABI. These machines use capillary electrophoresis, which is a significant advantage over slab-gel based technologies. Rather than risking inconsistency as a result of pouring slab-gels, a simple capillary array is fit into the slots available. In addition, the arrays are guaranteed for 100 runs (rather than one slab per run) and there is no sample loss (unlike a slab-gel).

 

The fragments of DNA (and all other negatively charged molecules) are attracted to the platinum cathode electrode attached to the end of the capillary (16 capillaries in all), which is open and filled with Performance Optimized Polymer (POP). When current is applied, the DNA fragments are attracted to the capillary and travel through the polymer to the detection window and array housing.

 

As the fragments pass through a clear area in the capillary, the fluorophores are excited by a laser and emission is detected and recorded by a CCD camera (the 3130 series can detect up to five fluorophores). The ability to detect multiple fluorophores enables detection of a larger number of similar size DNA fragments.

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