The Illumina MiSeq System offers capabilities for small whole-genome sequencing, amplicon sequencing, gene expression profiling, small RNA analysis and 16s metagenomic sequencing in a compact instruement. Using a high throughput kit, MiSeq can produce as much as 15 Gb of output from 50 million paired end reads (at a read length of 300 bp).
The system also features an intuitive touch screen interface, plug-and-play reagent cartridges with RFID tracking, on-screen video tutorials, and step-by-step guides throughout each sequencing workflow. The Illumina sequencing systems utilize a well-established sequencing by synthesis (SBS) method in which fluorescently labeled nucleotide bases are detected as they are incorporated into DNA template strands.
All four reversible terminator-bound dNTPs are present in each sequencing cycle; this feature minimizes bias of incorporation and greatly reduces raw error rates.
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